1a.Objectives (from AD-416)
Develop alternatives to methyl bromide for the control of soilborne diseases of strawberry.
1b.Approach (from AD-416)
Field research to elucidate ecological, plant disease and weed dynamics in strawberry production. Documents NFCA with UC Davis. Replaces 5305-22000-012-09N (6/09).
The objective of the project is to develop a molecular marker system for identification and quantification of Verticillium dahliae from soil samples. A region of the genome was identified where differences were observed between V. dahliae and related species and PCR primers were developed. Real-time PCR detection techniques (both SYBR green and TaqMan chemistries) were validated for species-specificity. Work was conducted to optimize DNA extraction techniques from soil to inhibitors that can reduce the efficiency of PCR amplification and an internal control was developed to assess PCR amplification efficiency. Communication on the project status is done with the granting agency by e-mail, personal conversations and submission of written yearly progress reports to the University of California.