1a.Objectives (from AD-416)
To develop and apply marker assisted selection to genetics and plant breeding in cotton.
1b.Approach (from AD-416)
Skills, knowledge, ability, and experience in developing and using DNA markers in genetics and plant breeding will be used to utilize molecular genetics knowledge in cotton genetics and plant breeding research. Research will involve finding markers associated with important traits in cotton and utilization of these markers to improve approaches to breeding new and useful traits into cotton.
Research to determine simple sequence repeat (SSR) markers for root-knot nematode and for reniform nematode was conducted and completed. Markers on chromosome 11 (CIR 316-201) and chromosome 14 (BNL3661-185) were discovered for root-knot nematode from the Clevewilt 6 and Mexico Wild sources. Markers for two quantitative trait loci (QTL) on chromosome 21 and one QTL on chromosome 18 were discovered for reniform nematode from the GB713 source. One QTL on chromosome 21 is flanked by BNL1551-162 and GH132-199. A second QTL on chromosome 21 is flanked by BNL-4011-155 and BNL3279-106. The QTL on chromosome 18 is flanked by BNL1721-178 and BNL569-131. Work was completed. This project was monitored by phone conversations and joint publication of results.