Start Date: May 21, 2009
End Date: May 20, 2013
Project #1: 1) develop a saturated genetic and physical map of the chromosome 2(2H) FHB QTL region with emphasis on bin 10; 2) test mutants selected in previous grant years for resistance to FHB. Make appropriate crosses to integrate the earliness, semi-dwarf and 6-rowed traits into a single FHB resistant parent for use in breeding; 3) screen the 2007 mutagenized population of CI4196 for additional mutants particularly 6-rowed and intermedium types; and 4) continue evaluating for FHB resistance and improving recombinant lines. Project #2: 1. Sequence 24 BAC clones from the chr. 2H bin10 region in the first year; 2. Analyze the sequence to identify putative genes; 3. Confirm and extend the BAC contigs as needed; 4. Sequence 24 additional BAC clones in the second year; 5. Repeat steps 2 and 3; and 6. Analyze putative resistance gene orthologs in CI4196.