Project Number: 5348-21430-005-03
Project Type: Specific Cooperative Agreement

Start Date: Aug 14, 2009
End Date: Jun 30, 2014

Objective:
Objective 1: Identify and characterize unique resistance sources from landraces that are amenable for introgression into adapted cultivars. Multiple new sources of resistance are important because resilient resistance to rusts has been problematic due to the rapid evolution of new virulent races within the fungi, this means that continuous efforts are essential to stay ahead of new emerging virulence. Objective 2: Identify molecular markers to facilitate both incorporation of resistance into adapted cultivars, and for marker assisted selection (MAS).

Approach:
Germplasm from the USDA-ARS National Small Grains Germplasm Collection (NSGC) will be screened utilizing Amplified Fragment Length Polymorphism (AFLP) and Simple Sequence Repeat (SSR) markers for cladistic analysis. Caldistic analysis will be used to determine if stem rust resistant germplasm arose from a monophyletic origin or if multiple sources of novel resistance are represented within the germplasm collection. Develop genetic mapping populations to characterize resistance alleles to Ug99 in resistant cultivars. Documents SCA with Washington State University.