ISOLATION AND IDENTIFICATION OF MOSQUITO REPELLENT CONSTITUENTS FOUND IN MALE INFLORESCENCES OF BREADFRUIT AND/OR BREADNUT
Natural Products Utilization Research
2011 Annual Report
1a.Objectives (from AD-416)
The objective of this cooperative research project is to isolate and identify any compounds with mosquito repellent properties found in male inflorescences of breadfruit and/or breadnut (Artocarpus altilis Parkinson (Fosberg), A. camansi Blanco, and A. mariannensis Trécul.)
1b.Approach (from AD-416)
Initial tests have shown significant mosquito repellent activity from selected extracts of male breadfruit and/or breadnut inflorescences. The research will be conducted with the following approaches:
1) Bioassay-guided fractionation will be used to isolate the compounds responsible for this property.
2) Purification and identification of the active constituent using nuclear magnetic resonance (NMR), gas chromatography/mass spectrometry (GC/MS) and other analytical techniques as necessary.
3) Evaluation of the potential of isolated phytochemicals and their derivatives as novel mosquito repellents or feeding deterrents in various bioassays.
This serves as the final report as all objectives and goals of this project have been met and the project is complete. The mosquito repellent compounds present in the male inflorescences of breadfruit and/or breadnut have been identified. The three most active compounds identified were capric acid, undecanoic acid and lauric acid. All compounds are as active as DEET in the K & D Bioassay system used in this study. We are still writing the manuscript and plan to submit it to Economic Botany by this summer. No intellectual property was discovered in this project since the bioactive constituents are fatty acids with previously reported activities. Work was monitored via email and telephone calls.