ISOLATION AND IDENTIFICATION OF MOSQUITO REPELLENT CONSTITUENTS FOUND IN MALE INFLORESCENCES OF BREADFRUIT AND/OR BREADNUT
Natural Products Utilization Research
2010 Annual Report
1a.Objectives (from AD-416)
The objective of this cooperative research project is to isolate and identify any compounds with mosquito repellent properties found in male inflorescences of breadfruit and/or breadnut (Artocarpus altilis Parkinson (Fosberg), A. camansi Blanco, and A. mariannensis Trécul.)
1b.Approach (from AD-416)
Initial tests have shown significant mosquito repellent activity from selected extracts of male breadfruit and/or breadnut inflorescences. The research will be conducted with the following approaches:
1) Bioassay-guided fractionation will be used to isolate the compounds responsible for this property.
2) Purification and identification of the active constituent using nuclear magnetic resonance (NMR), gas chromatography/mass spectrometry (GC/MS) and other analytical techniques as necessary.
3) Evaluation of the potential of isolated phytochemicals and their derivatives as novel mosquito repellents or feeding deterrents in various bioassays.
The objective of this cooperative research project was to isolate and identify any compounds with mosquito repellent properties found in male inflorescences of breadfruit and/or breadnut (Artocarpus altilis Parkinson (Fosberg), A. camansi Blanco, and A. mariannensis TrÃ©cul.) Preliminary studies indicated the efficacy of this traditional practice and indicate that extracts made from male inflorescences of Artocarpus altilis and the smoke produced when they are burned deter mosquitos from feeding in a live mosquito bioassay. The current study was undertaken to elucidate the underlying chemical constituents responsible for this putitive repellent activity. The bioactive constituents have been identified and a publication on the subject is being written. Methods used for monitoring or discussing research progress were by telephone call and email.