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United States Department of Agriculture

Agricultural Research Service

Research Project: SAFETY OF BIFIDOBACTERIUM LACTIS (BB12)SUPPLEMENTED YOGURT FOR ADULTS ON ANTIBIOTICS

Location: Diet, Genomics and Immunology Lab

2011 Annual Report


1a.Objectives (from AD-416)
To determine the impact of health-promoting components in foods including oral delivery of probiotic bacteria. In-house research demonstrated that Bb12 has a beneficial immune modulating effect on neonatal pigs with improved epithelial and changes in systemic immune markers. On the basis of these results, similar effects are predicted in humans. The Project Plan has one objective that directly relates to this agreement: To elucidate the mechanisms used by probiotic bacteria to improve respiratory and intestinal mucosal responses to allergens, and correlate intestinal micro-flora composition of pigs and humans with biomarkers of allergic and intestinal disease. The Cooperator is interested in the evaluation of the effects of Bb12 on adults given antibiotics as part of a Phase I study to demonstrate the safety of the strain and improve health outcomes. ARS will acquire nucleic acids from blood and fecal samples from treated subjects to detect specific bacterial genes and patterns of whole blood gene expression as was developed using the pig model. This information will be used by both ARS and the Cooperator to jointly develop studies that focus on the effects of Bb12 on the prevention of intestinal disease.


1b.Approach (from AD-416)
ARS will acquire nucleic acids from blood and fecal samples from treated subjects to detect specific bacterial genes and patterns of whole blood gene expression as was developed using the pig model. This information will be used by both ARS and the Cooperator to jointly develop studies that focus on the effects of Bb12 on the prevention of intestinal disease.


3.Progress Report

Georgetown University conducted a study to establish the safety of strawberry flavored yogurt supplemented with Bifidobacterium animalis subsp. lactis (B. lactis) strain Bb12; it was given to 40 adults for 10 days. Host colonization by probiotic bacteria was monitored by analyzing changes in commensal bacterial abundance and the level of Bb12 detected at time 0, 14, and 28 days after initiation of the study. Gene expression changes in peripheral blood were also determined. The data has been collected and is currently being analyzed. This agreement was monitored through email, visits, and telephone communication.


Last Modified: 12/22/2014
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