MOLECULAR CHARACTERIZATION OF NEMATODE AND FUSARIUM WILT RESISTANCE GENE-RICH REGION IN THE COTTON GENOME
Plant Stress and Germplasm Development Research
2010 Annual Report
1a.Objectives (from AD-416)
The objective of this research is to map and saturate cotton chromosomes 11 and 21 with existing and new EST and BAC-end derived SSR, STS, and SNP markers to discover and validate marker-associations with genetic control of nematode and wilt resistance (R) genes on diverse cotton populations with different genetic backgrounds.
1b.Approach (from AD-416)
To map and saturate cotton chromosome 11 (formerly LG A03) and its homoeologous chromosome 21 (formerly LG D02), we will mine the existing molecular marker resources (in-house and www.cottonmarker.org). Large numbers of microsatellite or SSR markers located in different cotton chromosomes are available for mapping. These molecular markers such as BAC-end sequence based SSR markers, and EST-based SNPs will be evaluated on different genetic backgrounds. Existing and new molecular-marker data from screened cotton populations segregating for pathogen resistant phenotype will be analyzed by statistical and genetic packages. Both qualitative and quantitative (QTL)-based mapping will be conducted to reveal markers associated with nematode and wilt resistance genes and their genetic positions in the R-gene cluster region. Pools and superpools of the BAC libraries will be screened with marker-primers and probes developed from R gene linked marker sequences (e.g. CIR316, BNL1231, and MUCS088) and from G. hirsutum cv., Maxxa BAC-end SSR sequences. Additional new markers will be generated by sequencing BAC clones which will be aligned in gene cluster regions. Several BAC clones will be sequenced, and their sequences will be analyzed for possible targets for R genes. Resistance genes/markers will be evaluated and validated for marker–R gene associations by screening the markers on additional populations from cotton crosses for which the progenies have been pathogen-resistance phenotyped. Documents NFCA with UC Riverside.
The agreement was established in support of objectives 4b and 4c of the in-house project, the goal being to map selected cotton chromosomes and identify genes responsible for resistance to root knot nematode and Fusarium wilt, both of which cause yield losses in cotton. Segments of DNA from the Acala Maxxa cotton cultivar were sequenced and compared to published databases for similarity to known disease resistance genes. The locations of sequenced segments were selected based on previous genetic analyses of resistance. These sequences were used to derive molecular markers for development as tools to speed efforts to breed cotton for nematode and Fusarium resistance. Activities during this project were documented through presentations at meetings of cotton growers, professional societies, and commodity groups, and through telephone contacts and site visits with collaborators.