1a.Objectives (from AD-416)
To identify new molecular markers.
1b.Approach (from AD-416)
Sequence cDNA libraries from diverse U.S. germplasm to identify new SNP alleles. Using bioinformatics identify useful SNP alleles. Using the allumnia SNP platform over 4000 SNPs will be mapped in multiple CAP mapping populations.
The objective of the Single Nucleotide Polymorphism (SNP) grant was to sequence relevant wheat cultivars, utilize the sequence information to identify new single nucleotide polymorphisms and develop SNP based molecular markers. Progress on this objective has exceeded expectations. Efforts coordinated by the western regional genotyping lab resulting in 1,824 wheat lines including 6 mapping populations being tested against 9,000 SNP. Results from the assay included 5,232 new polymorphic SNP markers of which 4,143 SNP can be placed on the 6 genetic maps. The resulting 9.5 million datapoints are currently being evaluated for their relevance in each breeding program and will soon be implemented in genome selection efforts for marker assisted selection.