Start Date: Jul 01, 2009
End Date: Sep 30, 2010
(1) Separate soft tissue material from bone material; determine the proximate composition and physical properties of both fractions. Combine amino acid and mathematical analyses to determine the identity and concentration of each of the major proteins. (2) Evaluate methods for the controlled hydrolysis of the protein in whole or fractionated MBM. Emphasize methods purported to inactivate BSE prions, but likely to scale up well and be economical. Evaluate the crosslinking of some hydrolysates. Determine several of the bulk functional properties of the treated protein and compare with those of other commercial proteins. (3) Develop an economical process for production of an MBM or component hydrolysate that matches or exceeds the desirable characteristics of other undefined nitrogen sources for low-cost, non-pharmaceutical industrial fermentation.