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United States Department of Agriculture

Agricultural Research Service

Research Project: Ultra-High Throughput Sequencing for Discovery and Characterization of Viral Genomes in Fire Ants Project Number: 6615-32000-044-07
Project Type: Trust

Start Date: Apr 01, 2009
End Date: Aug 31, 2013

Objective:
Use a metagenomics approach to identify new viruses infecting the fire ant Solenopsis invicta and subsequently use genomic tools and resources to study the biology (distribution, pathogenicity, etiology, molecular evolutionary history) of several of these viruses.

Approach:
1) Perform a large-scale EST study using 454 pyrosequencing technology to identify potential viruses infecting fire ants using bioinformatics tools and manual annotation of genes. 2) Confirm that newly discovered viruses occur within S. invicta and are not contaminants. 3) Sequence the entire genomes of newly discovered viruses. 4) Use an existing cDNA microarray to determine the host response to viral infections by comparing and identifying genes differentially expressed between virus-infected and uninfected fire ants. 5) Sequence multiple genomes of a single virus and multiple alleles of host genes that respond to viral infection and examine their molecular evolution.

Last Modified: 12/28/2014
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