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United States Department of Agriculture

Agricultural Research Service

Research Project: EVALUATION OF THLASPI AND CAMELINA ACCESSIONS

Location: North Central Regional Plant Introduction Station, Ames, Iowa

2012 Annual Report


1a.Objectives (from AD-416):
Cultivars of Camelina sativa have already been released and are being grown for feed, food and biofuel production. Thlaspi arvense, commonly known as pennycress, and other Thlaspi species are of interest for oilseed production because their biennial growth habit would fit into a crop production scheme where Thlaspi would be produced from October-early June, and followed by a different annual crop that would utilize the summer growing season. Neither of these Brassicaceae collections held by the NPGS has been thoroughly evaluated in a common location. Understanding the range of adaptive, phenotypic and compositional traits would enable researchers to better understand genotype x environmental interactions on traits of interest and to target accessions useful for domestication, crop production and end use.


1b.Approach (from AD-416):
In collaboration with the Ames PIRU and the Peoria NCAUR units, ISU will oversee the field and laboratory experiments as follows, and be responsible for loading all GRIN descriptor data into the GRIN system.

Descriptor evaluations will be collected in a replicated design for approximately 40 Camelina and 20 Thlaspi accessions, including appropriate Brassica benchmark accessions as checks, at two locations (Ames, IA and Peoria, IL).

1. Determination of vernalization (whether it is required for bolting) and germination requirements (Ames).

2. Phenological descriptors – emergence date, first, middle and last flower date, flower color, days to harvest in field conditions and under controlled conditions (growth chambers).

3. Morphological and growth descriptors - number of leaves at bolting, flower stalk height, plant height, uniformity, various silique, locule and seed descriptors.

4. Seed production descriptors – seed yield, 100 seed weight.

5. Chemical descriptors – fatty acid compositional profile, seed glucosinolate content, percent oil content, development of oil content during maturation.

6. Collection and posting of digital images of flowers, plants, siliques, and seeds. 7. Tissue collection on DNA cards (2009-2010) for future co-dominant SSR or SNP molecular marker analyses, not currently funded by this project.


3.Progress Report:

In-vivo and in-vitro trials were conducted to determine whether vernalization is a requirement for bolting (flowering) of pennycress (Thlaspi arvense) and camelina and their germination requirements (in Ames) with ISU collaborators. Evidence indicates that none of the camelina accessions tested have vernalization requirements, but variability for the trait exists in the pennycress collection. Phenological descriptors – emergence date, first, middle and last flower date, flower color, days to harvest in field conditions and under controlled conditions (growth chambers). These data were collected with collaborators in Ames, IA, Fargo, ND, and Peoria, IL. Morphological and growth descriptors - number of leaves at bolting, flower stalk height, plant height, uniformity, various silique, locule and seed descriptors – were collected in Ames and from surviving plots in Fargo, ND. Seed production descriptors – seed yield, 100 seed weight – were collected. Chemical descriptors – fatty acid compositional profile, seed glucosinolate content, percent oil content, development of oil content during maturation. These traits were analyzed in collaboration with the USDA-ARS-NCAUR in Peoria, IL. Collection and posting of digital images of flowers, plants, siliques, and seeds. Digital images have been collected, and are progressively posted to the GRIN database by ISU curatorial staff. Tissue collection on DNA cards (2009-2010) for future co-dominant SSR or SNP molecular marker analyses, not currently funded by this project. Tissue was collected, DNA was extracted, and SSR markers were tested for polymorphisms.


Last Modified: 11/26/2014
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