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United States Department of Agriculture

Agricultural Research Service

Research Project: EVALUATION, BREEDING, AND GENOMICS OF FHB RESISTANCE IN WHEAT AND BARLEY Project Number: 0500-00053-003-22
Project Type: Grant

Start Date: Jun 17, 2009
End Date: Jun 16, 2014

Objective:
Project Title 1 - Breeding and Genomics of FHB Resistance in Soft Red Winter Wheat: This project will deploy both conventional and molecular technologies to accelerate progress in breeding and genomics research on Fusarium head blight (FHB) resistance. Project Title 2 - Screening Hordeum Germplasm for Resistance to Fusarium Head Blight and DON Accumulation: 1) evaluate and validate putative resistance sources of Hordeum for reaction to FHB; in replicated experiments in an inoculated and mist-irrigated scab nursery and; 2) make crosses between the resistant accessions and adapted breeding lines as part of a pre-breeding program.

Approach:
Project Title 1 - Marker assisted selection (MAS) and breeding strategies using newly identified markers and known diagnostic markers for major QTL from diverse genetic backgrounds will be used in genotypic characterization and selection of parents, progeny and pure lines to implement pyramiding of QTL conferring complementary types of FHB resistance. Partial sequence information of each QTL will be obtained and compared and analyzed through NCBI and other database searches for putative candidate genes for FHB resistance. These studies will facilitate more efficient deployment and selection of specific QTL regions and different types of FHB resistance. Project Title 2 - We propose to evaluate about 60 to 100 diverse Hordeum accessions for reaction to FHB. The accessions selected for this study will be mostly from the N. I. Vavilov All-Russian Scientific Research Institute of Plant Industry [VIR], International Center for Agriculture in the Dry Areas [ICARDA], and Plant Genetic Resources Canada [PGRC] genebanks and targeted to represent unique germplasm not screened in previous tests. Resistant winter habit barley accessions will be evaluated in replicated FHB nurseries at Blacksburg, Virginia.

Last Modified: 12/22/2014
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