1a.Objectives (from AD-416)
In a collaborative effort to evaluate the effects of a micronutrient supplement in children, we will provide consultation in the protocol development for the collection of blood from 300 children at baseline and 6 months post feeding and perform the assays of nutritional status biomarkers.
1b.Approach (from AD-416)
We will analyze blood for zinc, B12, folate, retinol and serum transferrin receptors. If more serum is available, the following biomarkers will be performed in the following order: vitamin D, riboflavin and CRP.
From January 2009 to December 2010, a lipid based nutrient supplement vs. a standard complementary food was provided for 6 months to 400 children in rural Honduras. WHNRC provided advice on sample collection and handling, and in FY2011 analyzed 1200 samples for zinc, vitamin B12, folate and red blood cell riboflavin; 800 for retinol; and 1000 for transferrin receptors, CRP, and AGP. An abstract and preliminary data were presented at the International Lipid Supplement Nutrition Study in Washington DC, April 2012, showing an improvement in B12 status with supplementation. We are currently assisting with the data analysis which is ongoing at the University of North Carolina, Chapel Hill.