2010 Annual Report 1a.Objectives (from AD-416) (1) Establish baseline metabolic profiles of commercial citrus products through the use of modern analytical methods, and (2) Determine the influence of cultivar variety, environment and plant health on the metabolite composition by comparing the profiles obtained for these samples to those obtained for other citrus products, and (3) Identify diagnostically significant metabolites. 1b.Approach (from AD-416) Pepsico will provide the majority of the samples for this study. Additional samples may be obtained from commerical citrus growers or from citrus variety collections. Samples will be processed into juice and stored as such until analyzed by GC-MS, LC-MS, HPLC and NMR. Additional analyses will be performed as needed. Initial data analysis and the subsequent statistical analysis will be conducted at the Western Regional Research Center, Albany, CA. Documents trust with Pepsico. Log 37997. 3.Progress Report This agreement was established in support of Objective 3 of the in-house project, the goal being to characterize the phytochemicals found in citrus and to develop strategies to modify and/or enhance the character or amount of these compounds in order to improve the quality of citrus, increase its consumer acceptance and to optimize its nutritional value. During this reporting period, research efforts on this project focused on acquiring the necessary samples, establishing sampling handling procedures and developing GC-MS and NMR analytical methods. Progress in this projected is monitored through periodic reports and correspondence.