2011 Annual Report 1a.Objectives (from AD-416) (1) Establish baseline metabolic profiles of commercial citrus products through the use of modern analytical methods, and (2) Determine the influence of cultivar variety, environment and plant health on the metabolite composition by comparing the profiles obtained for these samples to those obtained for other citrus products, and (3) Identify diagnostically significant metabolites. 1b.Approach (from AD-416) Pepsico will provide the majority of the samples for this study. Additional samples may be obtained from commercial citrus growers or from citrus variety collections. Samples will be processed into juice and stored as such until analyzed by GC-MS, LC-MS, HPLC and NMR. Additional analyses will be performed as needed. Initial data analysis and the subsequent statistical analysis will be conducted at the Western Regional Research Center, Albany, CA. 3.Progress Report This agreement was established in support of the objectives of the previous in-house project, 5325-41430-010-00D, the goal being to characterize the phytochemicals found in citrus and to develop strategies to modify and/or enhance the character or amount of these compounds in order to improve the quality of citrus, increase its consumer acceptance and to optimize its nutritional value. During this reporting period, research efforts on this project focused on conducting gas chromatography mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) analyses and determining the limonin content initially present in the samples and following storage. Progress in this project is monitored through periodic reports, correspondence, and quarterly teleconferences.