2009 Annual Report 1a.Objectives (from AD-416) (1) Establish baseline metabolic profiles of commercial citrus products through the use of modern analytical methods, and (2) Determine the influence of cultivar variety, environment and plant health on the metabolite composition by comparing the profiles obtained for these samples to those obtained for other citrus products, and (3) Identify diagnostically significant metabolites. 1b.Approach (from AD-416) Pepsico will provide the majority of the samples for this study. Additional samples may be obtained from commercial citrus growers or from citrus variety collections. Samples will be processed into juice and stored as such until analyzed by GC-MS, LC-MS, HPLC and NMR. Additional analyses will be performed as needed. Initial data analysis and the subsequent statistical analysis will be conducted at the Western Regional Research Center, Albany, CA. Documents trust with Pepsico. Log 37997. 3.Progress Report For this project we have continued our analysis of Satsuma mandarin orange juices obtained from 10 different orchards located in Placer County and expanded the scope of the study to include metabolite profiling and molecular analyses. Profiling experiments target carotenoids, limonoids and volatile juice components. Molecular analyses focused on the expression of enzymes in the synephrine biosynthetic pathway.