Start Date: Mar 01, 2009
End Date: Feb 28, 2010
Eleven (11) of the most promising recombinant M. paratuberculosis proteins (MAP0865, MAP0857c, MAP3817c, MAP3155c, MAP2077c, MAP3761c, MAP4014, MAP2166c, MAP3735c, MAP0900 and MAP1272c) will be evaluated by ELISA using a set of well-defined Johne’s disease-infected cattle sera as well as sera from control cattle. Additional recombinant proteins may be selected and screened based on preliminary data on antigenicity and specificity. Methodologies that will be used in this project include genomic and bioinformatics analysis techniques, immunological assays (Western blot and ELISA), and molecular cloning, as well as recombinant protein expression and purification, diagnostic test development and optimization. Proprietary PRIONICS ELISA technology will be used in this project, as well as a process for mass production of recombinant proteins developed by ARS.