Location: Crop Improvement & Utilization Research
2010 Annual Report
The agreement was established in support of Objective 3 of the in-house project, to develop tools to refine down-regulation technologies for metabolic manipulation as well as improve design characteristics of pathogen-resistance transgenes.
The goal of this project is to use next generation sequencing to understand and elucidate large and complex potato genomes and use this information to analyze and develop tools to refine down-regulation technologies for metabolic manipulation as well as improve design characteristics of pathogen-resistance transgenes.
The wild potato relative (Solanum bulbocastanum) is the ongoing subject of next generation sequencing with an Illumina Genome analyzer and to date 3.4 Gigabytes of sequence, or roughly 3x genome coverage, has been obtained for analysis. Preliminary assemblies have been mostly limited to high copy plastid and mitochondrial genomes, and sequence information will be analyzed further upon the release of the draft potato genomes (S. phureja and S. tuberosum) from the Plant Genome Sequencing Consortium.
Using Next generation sequencing, small RNAs (sRNA) of potato leaves have been sequenced for two varieties of cultivated potatoes (controls) and for four transgenic lines containing inverted hairpin structures designed to produce small inhibitory RNA (siRNA) constructs directed towards the potato virus Y (PVY). Some of the transgenic lines showing positive phenotypes for disease resistance lines were successfully analyzed and demonstrated to have unique and strong siRNA production associated with viral resistance.
Participants in this project have monitored the research through exchange of data, electronic communications, phone calls, and personal meetings.