2010 Annual Report 1a.Objectives (from AD-416) To intrgress novel SCN-restantance genes into high-yielding soybean germplasm of early maturity groups (I, II and III) possessing resistance to other economically important disease with adequate agronimic performance. 1b.Approach (from AD-416) Populations and lines will be developed using different sources of SCN resistance identified by ARS. The cross will be made between new sources or lines of SCN resistance and experimental elite ISU lines developed by Cooperator. Selections include greenhouses bioassays and marker assited breeding for SCN resistance and field evaluations for superior performance. 3.Progress Report Introgression of novel genes conferring resistance to soybean cyst nematode (SCN) in soybean germplasm is a collaborative research project funded by the Iowa Soybean Association via Iowa State University. Additional Crosses were made for broadening resistance in soybean of early maturity groups primarily II and III which are adapted to the Midwest area. Genetically diverse sources identified at ARS, Jackson, TN worksite were used to make crosses including (PI438489B, PI22897 and PI437655). Resistance genes from diverse sources are transferred into two high yielding elite lines, A95-684043 and LD00-3309 from the Midwest region. The elite lines are susceptible to SCN. Progenies are being screened for resistance to Races 3 and 4 in the greenhouse. DNA isolated from the selected progenies is being used to confirm for resistance to SCN through DNA markers tagged to resistance. ADODR primarily monitored project activities through meetings, conference calls, emails and site visits.