Project Number: 3640-21000-028-01
Project Type: Specific Cooperative Agreement

Start Date: Jul 13, 2009
End Date: May 23, 2013

Objective:
Conduct research to identify, characterize, and determine the function of genes involved in nitrogen fixation, nitrogen assimilation, phosphorus acquisition, and root development in Lupinus, Medicago, and Phaseolus.

Approach:
1. Prepare cDNA libraries from various plant organs of the three legume species. Develop genomic libraries of the three species. 2. Use high throughput sequencing to evaluate transcriptome. 3. Sequence genes and prepare antibodies to the gene products. 4. Evaluate gene expression via RNA blots, immunoblots, enzyme assays, and in situ hybridization. 5. Characterize potential promoter elements for important genes related to the processes cited above. 6. Modify expression of relevant genes via overexpression, antisense, and/or RNAi approaches. BSL-1; Re-Certified June 12, 2007.