1a.Objectives (from AD-416)
Develop a whole genome association platform for sorghum and identify SNP markers associated with aluminum tolerance.
Identify polymorphisms associated with Al tolerance in maize.
Develop innovative methods for analysis of root architecture and the role it plays in acid soil tolerance.
Determine the genetic architecture of high Al tolerance in maize based on previously identified Al tolerance QTL and orthology to sorghum AltSB.
Improve Al tolerance in maize by introgressing AltSB homologs co-localized with Al tolerance QTLs as well as other Al tolerance QTLs into maize tropical breeding lines.
Assess the yield advantage of Al tolerant maize in Kenyan environments and begin to investigate the contribution of Al tolerance to drought tolerance.
1b.Approach (from AD-416)
We will use a combination of association and QTL mapping to identify and verify novel sorghum and maize Al tolerance genes, and use marker assisted breeding to introgress the best alleles of these genes into maize and sorghum for generating cereal crops better suited for cultivation on acidic, Al toxic soils.
In 2011, progress on this agreement involved work on sorghum and maize drought tolerance in the field. Thus we concluded scoring yield in the field under well watered and drought conditions, and are conducting QTL analysis of the data. This is enabling us to identify specific regions of the sorghum genome that contain genes that confer the ability for sorghum to have improved yields under drought. These findings may lead to the identification of genetic markers that can be used to improve sorghum drought tolerance through molecular breeding.