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United States Department of Agriculture

Agricultural Research Service

Research Project: NESTED ASSOCIATION MAPPING TO IDENTIFY YIELD QTL IN DIVERSE HIGH YIELDING ELITE SOYBEAN LINES

Location: Soybean Genomics and Improvement

Project Number: 8042-21000-263-30
Project Type: Reimbursable

Start Date: Apr 02, 2009
End Date: Dec 31, 2012

Objective:
The discovery of genes or quantitative trait loci (QTL) that control yield per se in elite soybean germplasm.

Approach:
The high yield reference genotype, IA3023, was crossed to 25 diverse accessions to create 25 populations with 200 recombinant inbred lines (RILs) each. Thus, each population shares the reference genotype as one of the parents. The 5000 RILs will be phenotyped by collaborators and genotyped with SNPs as per a typical QTL population analysis. The 25 diverse accessions will be extensively genotyped or sequenced which allows for high marker density which can be extrapolated to all the RILs in the 25 populations, thus greatly increasing the power to detect and fine map QTL. Using this approach, essentially all SNPs present in all 5000 RILs can be determined and the most common QTL among the 25 diverse lines can be discovered.

Last Modified: 11/25/2014
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