Location: Plant Gene Expression Center Albany_CA
2010 Annual Report
ARS scientist demonstrated that the BOP1 and BOP2 regulatory proteins control leaf formation by suppressing KNOX1 and YABBY transcription factor activity at the base of the organ. Results were reported in the peer-reviewed journal Genes & Development (Ron, M. et al. 2010. Genes & Dev 24: 1010-1021).
ARS scientist identified a maize mutant with increased glucan in the cell wall, an impediment to plant feedstocks and identified the tasselsheath4 gene that regulates leaf size which is critical for improving biofeedstocks. Research results were published in six publications.
ARS scientist demonstrated that mutants without normal circadian rhythms could not distinguish between moderate and warm temperatures and that maize genes with expression regulated by the circadian clock is integral to maize photosynthesis, carbohydrate metabolism, cell wall synthesis, and phytohormone production. Research results published in Genome sequencing and analysis of the model grass Brachypodium distachyon. Nature. 463:763-768.
Demonstrated that ARS scientist analyzed plants regenerated from calluses and showed that site specific integration mediated by the Bxb1 recombination system occurred at a ~10% frequency. This is practical for commercial developers to obtain site-specifically integrated transgenes, and will help speed the development of more precisely engineered transgenic varieties. Research results were published in The phiC31 Recombinase Demonstrates Heritable Passage of Site-specific Genomic Excision in Arabidopsis. BioMed Central (BMC)Biotechnology.Available:doi:10.1186/1472-6750-10-17.
An ARS scientist concluding 10 years of research on the function of the ACC synthase enzymes encoded by 9 genes in Arabidopsis. The results revealed a critical role for ACC and were published in A Combinatorial Interplay Among the 1-Aminocyclopropane-1-carboxylate Isoforms Regulates Ethylene Biosynthesis in Arabidopsis thaliana. Genetics. doi:10.1534/genetics.109.107102
An ARS scientist investigated the functional and evolutionary properties of three pathogen disease resistance loci of wild Solanum species using comparative genomics. Results were published Identification of miniature inverted-repeat transposable elements (MITEs) and biogenesis of their siRNAs in the Solanaceae: New functional implications for MITEs. Genome Research. 19:42-56 and Inferred origin of several Native American potatoes from the Pacific Northwest using SSR markers. Euphytica. 174:15-29.
Research at the PGEC provides an essential knowledge base for biotechnology's impact on agriculture. The knowledge has moved from determining the function of single genes to elucidation of entire networks. This information can be used to understand the function of developmental processes in crop processes.
Monitoring of activities is carried out by weekly seminars and monthly meetings.