Location: Plant Gene Expression Center Albany_CA
2009 Annual Report
An ARS scientist determined that the hormone gibberellin is directly regulated by the KNOTTED1 transcription factor and that the regulatory sequences of the GA-2-oxidase gene are conserved in the grasses. She has also identified a number of other direct targets using chromatin sequencing.
An ARS scientist identified a substantial number of maize transcripts under circadian regulation and discovered several likely maize clock components. Microarray analysis showed 10% of maize transcripts exhibit a circadian rhythm; a clear illustration of the maize circadian oscillator’s broad transcriptional regulatory purview.
An ARS scientist showed that mutants which carry truncated versions of a protein disulfide isomerase have embryo sac maturation and disrupted pollen tube guidance, whereas gene knockouts in this gene had no effect..
An ARS scientist analyzed plants regenerated from calluses generated in FY08 and showed that site specific integration mediated by the Bxb1 recombination system occurred at a ~10% frequency. This is practical for commercial developers to obtain site-specifically integrated transgenes, and will help speed the development of more precisely engineered transgenic varieties.
An ARS scientist has discovered a novel complexity in phytochrome (phy)-mediated light signaling, whereby bHLH signaling partners function as transcription factors in regulating early gene-expression, but as direct feedback modulators of phy-protein abundance in regulating later visible seedling morphogenesis.
An ARS scientist is concluding 10 years of research on the function of the ACC synthase enzymes encoded by 9 genes in Arabidopsis. The results have revealed a critical role for ACC and is soon to be published.
An ARS scientist investigated the functional and evolutionary properties of three pathogen disease resistance loci of wild Solanum species using comparative genomics.
Research at the PGEC provides an essential knowledge base for biotechnology's impact on agriculture. The knowledge has moved from determining the function of single genes to elucidation of entire networks. This information can be used to understand the function of developmental processes in crop processes.
Monitoring of activities is carried out by weekly seminars and monthly meetings.