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United States Department of Agriculture

Agricultural Research Service

Research Project: MANURE-BORNE E. COLI FATE TRANSPORT IN AGRICULTURAL FIELDS AND VEGETATED FILTER STRIPS

Location: Environmental Microbial and Food Safety Laboratory

2011 Annual Report


1a.Objectives (from AD-416)
To develop comprehensive experimental data sets for testing and improving manure-borne pathogen fate and transport models suitable for nutrient management planning and environmental risk assessment.


1b.Approach (from AD-416)
Field experiments will be performed at the OPE3 field site and the Patuxent runoff site. to (a) evaluate the effect of the buffer strip status on its retention efficiency, (b) develop model parameters and evaluate model performance, (c) evaluate the phosphorus in runoff as a natural indicator of E. coli. The edge-of-the-field conditions will be simulated at the Patuxent site where the manure suspensions will be maniflolded to the simulated vegetated buffer strips with varying soil type, initial soil moisture content, vegetation status, and slope. The rainfall will be simulated to cause runoff that will be collected at the bottom of the strips. Concentrations of E. coli and P will be measured in the runoff transport. Appropriate complementary soil, hydrologic, and microbial measurements will be carried out. The field-scale transport will be studied at the OPE3 site after manure application in accordance with Maryland agronomic rates. E. coli contents will be monitored in manure, in soil, in runoff using automated samplers, in groundwater, and in the first order creek separated from the field with a riparian zone. All experiments will be performed with participation of USDA employees. Cooperator will participate in modeling, data analysis, and publication of results.


3.Progress Report

Field experiments were performed at the OPE3 field site and the Patuxent runoff site to (a) evaluate the effect of the buffer strip status on its retention efficiency, (b) develop model parameters and evaluate model performance. Appropriate complementary soil, hydrologic, and microbial measurements were carried out. The field-scale transport were be studied at the OPE3 site after manure application in accordance with Maryland agronomic rates. E. coli contents were monitored in manure, in soil, in runoff using automated samplers, in groundwater, and in the first order creek separated from the field with a riparian zone. All experiments were performed with participation of USDA employees. The important result of this work consisted in the demonstration of the need to include the kinetic energy of rain along with the rainfall intensity in the models of bacteria release, transport and retention in vegetated buffer strips and pasturelands.

The progress of the work has been monitored by monthly meetings with the UMD counterparts, and weekly group meetings.


Last Modified: 7/28/2014
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