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United States Department of Agriculture

Agricultural Research Service

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Research Project: POSITIONAL CANDIDATE GENES FOR RESISTANCE TO MAREK'S DISEASE BY SCREENING FOR MAREK'S DISEASE VIRUS MEQ-REGULATED GENES

Location: Avian Disease and Oncology Laboratory

Project Number: 5050-31320-009-01
Project Type: Reimbursable

Start Date: Jan 01, 2009
End Date: Dec 31, 2012

Objective:
1. Identify MDV Meq and c-Jun binding sites in the chicken genome. 2. Identify differentially expressed genes following MDV-reactivation in MSB-1 cells. 3. Determine if differentially expressed genes identified in objective 2 also show differentially expression between MD tumors and CD4+ T cells. 4. Identify differentially expressed genes as a function of genetic resistance status or the presence of Meq.

Approach:
1. Utilize chromatin immunoprecipitation (ChIP) using Meq or c-Jun antibodies and determine the DNA sequences via Solexa paired-end reads. 2. Isolate RNA from MSB-1 cells at time 0 and following MDV reactivation, and run on Affymetrix Chicken microarrays. 3. Compare by qRT-PCR the relative expression of key genes between MD tumors and CD4+ T cells. 4. Isolate RNA from line 6 (MD resistant) and line 7 (MD susceptible) CEF at various time before and after infection with either MDV or MDV lacking Meq, and run on Affymetrix Chicken microarrays.

Last Modified: 11/26/2014
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