1a.Objectives (from AD-416)
We will expand close working relationships among breeders, extensionists, and growers of major Alliums in the U.S. to evaluate germplasms for prioritized pest resistances (thrips and Iris Yellow Spot Virus) and lay the foundation for the long-term translational genomics of the Allium vegetables. Workshops will be held at regional onion meetings.
We will evaluate in the field onion populations for resistance or tolerance to thrips and Iris Yellow Spot Virus. Resistance or tolerant germplasms will be released to the onion breeders in the public and private sectors.
1b.Approach (from AD-416)
Work with extension professionals to empower growers to complete on-farm evaluations for resistances or tolerances to thrips and Iris Yellow Spot Virus. Self-pollinate and testcross selected plants and return seed for validation of phenotypes; Deliver validated germplasms to private and public sector breeders; Develop workshops for public and private-sector researchers, students, and regional grower and consumer groups for onion to illustrate the usefulness of genomics to solve high-priority research goals.
Onion cultivars from commercial entities and germplasm entries from the USDA Plant Introduction Station in Geneva, NY were evaluated in field plots for responses to thrips and subsequent infection by Iris yellow spot virus (IYSV). Agronomic information (leaf color, axil pattern, maturity, bulb size) and thrips (adults and larvae) counts were taken on plants at 3 stages of vegetative or reproductive development and compared to incidence (% of plants infected) and severity (scale of 0 to. 4)of IYSV. Individual plants or populations with putative tolerances to IYSV and/or thrips will be marked for separate harvest and bulbs were sent Wisconsin for controlled pollinations. Seed will be returned to Colorado to validate phenotypes. Project is monitored by conference calls every two months.