2012 Annual Report
1a.Objectives (from AD-416):
We will significantly increase the numbers of robust single nucleotide polymorphisms (SNPs) and develop a high through-put genotyping platform for onion. Workshops will be held at regional and national Allium meetings.
1b.Approach (from AD-416):
Develop a detailed genetic map for onion:
Identify simple single nucleotide polymorphisms (SNPs) in onion;
Develop high throughput SNP genotyping platform for onion; and
Develop workshops for public and private-sector researchers, students, and regional grower and consumer groups for onion to illustrate the usefulness of genomics to solve high-priority research goals.
Random sequences from two complementary deoxyribonucleic acid (cDNA) libraries of onion were assembled and annotated. Sequences were prepared for submission to public databases. A total of 4,734 single nucleotide polymorphisms (SNPs) were identified in 1,677 expressed sequences between two inbred onion populations. Of these, 1256 on independent cDNAs were randomly selected that were amenable for genotyping using the Kaspar platform.
This research relates to Objective 1, Determine the genetic basis of and initiate selection for carrot, onion, cucumber, and melon quality attributes influencing human nutrition and health, disease resistances, and yield and quality components, and stress tolerance in cucurbits, and perform field performance and quality trials; Objective 2, Utilize current biotechnology to discover and evaluate genetic variation and to map agriculturally important traits in Allium, Cucurbit, and Daucus germplasm, and to develop genetic and breeding stocks.