TRANSLATIONAL GENOMICS OF ONION FOR PRIORITIZED PEST RESISTANCES
Vegetable Crops Research Unit
2009 Annual Report
1a.Objectives (from AD-416)
Extension: We will expand close working relationships among breeders, extensionists, and growers of major Alliums in the US to evaluate germplasms for prioritized pest resistances and lay the foundation for the long-term translational genomics of the Allium vegetables. Workshops will be held at regional and national Allium meetings.
Research: We will significantly increase the numbers of robust single nucleotide polymorphisms (SNPs) and develop a high through-put genotyping platform for onion. We will tag chromosome regions controlling prioritized disease-resistance traits of onion and transfer these tools to the private sector.
1b.Approach (from AD-416)
Develop a detailed genetic map for onion:
o Identify simple single nucleotide polymorphisms (SNPs) in onion;
o Develop high throughput SNP genotyping platform for onion;
Work with extension professionals to empower growers to complete on-farm evaluations for resistances or tolerances to prioritized diseases and pests; Self-pollinate and testcross selected plants and return seed to the extension-grower collaborators for validation of phenotypes; Deliver validated germplasms to private and public sector breeders; Exploit association genetics to tag grower-selected resistances. Develop workshops for public and private-sector researchers, students, and regional grower and consumer groups for onion to illustrate the usefulness of genomics to solve high-priority research goals.
Specialty Crops Research Initiative.
Project was initiated in September 2008. Subcontracts with co-Principal Investigators were finalized and grant funds distributed. The research group held a planning meeting at the National Allium Research Conference in Georgia in December of 2008. Research by each of the collaborating institutions is described below. Onion inbreds B5225B and OH-1 were grown, vernalized in the cold, and tissues delivered to J. Craig Venter Institute (JCVI) for isolation of Ribonucleic acids (RNAs). Onion F2 families segregating for the semi-glossy phenotype were developed. Over 400 gynogenic haploids were extracted from F1 plants from the cross of OH-1 by B5225B for mapping of molecular markers. Project is monitored by conference calls every two months.