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United States Department of Agriculture

Agricultural Research Service

Research Project: Introgress Gossypium Barbadense L Alleles Into Elite G. hirsutum Germplasm Via Random Mating

Location: Genetics and Sustainable Agriculture Research Unit

2009 Annual Report

1a.Objectives (from AD-416)
To improve the utility of G. barbadense chromosome substitution lines for G. hirsutum breeding. To develop and release to the public a random mated population(s) that has unique combinations of alleles from the two species. To develop a breeding scheme that facilitiates the recombination of alleles from G. barbadense and G. hirsutum.

1b.Approach (from AD-416)
Seventeen CS-B lines have been crossesd to five elite cultivars from different commercial breeding programs in the U.S. ('DP 90', 'SureGrow 747', 'PSC 355', ST474, and FM 966). The CS-B lines ae CS-B01, CS-B02, CS-B04, CS-B06, CS-B07, CS-B16, CS-B17, CS-B18, CS-B25, CS-B05sh, CS-B11sh, CS-B12sh, CS-B14sh, CS-B15sh, CS-0B22sh, CS-B22Lo, and CS-B26Lo. The designations sh and Lo indicate that only the short or the long arm of a chromosome from G. barbadense is in the CS-B line. We will begin the random mating with two or three F1 hybrids from each of these 17 CS-B lines with two or three of the five cultivars. We will use the bulk pollen methodology of Miravalle to accomplish random mating, thus we will have 51 familes many of which will be half-sibs. The F1 hybrids will have one of five elite cultivars as one parent and a particular CS-B line as the other parent. From the cultivar parents we expect to gain adaptability, yield, and other necessary properties. From the CS-B parent we expect to gain alleles from particular chromosomes or chromosome arms from G. barbadense. These chromosome or chromosome arms should contain many alleles for fiber quality because of the very high fiber quality of the G. barbadense parent.

3.Progress Report

Cycles C0 and C1 of random mating were completed. The ADODR monitored this project by weekly contact with the scientist assigned to this project. The ADODR is also one of the principle scientists on the project. Quarterly reports were prepared for Cotton Incorporated.

Last Modified: 11/29/2015
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