2013 Annual Report
1a.Objectives (from AD-416):
To improve Foot-and-Mouth Disease Virus (FMDV) vaccine potency and duration of immunity, we will study the cellular immune response to infection and the ability to refine the killed virus vaccine for FMDV or the recombinant empty capsid vaccine. The objective of this agreement is to analyze T cell responses to FMDV infection in swine and cattle.
1b.Approach (from AD-416):
The state of the art for analysis of the cellular immune response of T lymphocytes to vaccination is: identification of T cell epitopes, mapping of those epitopes to the presenting transplantation antigens known as histocompatibility complex (MHC) molecules and developing MHC tetramers for tracking T cell responses. This technology is used extensively in laboratory mice but is expensive in a genetically diverse human population. Even given the expense, the technology is being applied in the clinic more and more often. Our preliminary studies indicate common cattle breeds, such as Holstein, can be analyzed using this technology.
The University of Copenhagen will generate the information required to design and test MHC tetramers for analysis of cellular immune reponse to Foot-and-Mouth Disease Virus(FMDV). ARS, PIADC will utilize this information to analyze responses of cattle and swine to vaccination and FMDV infection.
Synthesized 8 class I major histocompatibility complex (MHC) proteins; N*01901, N*01201, N*00801,N*02401, N*01701,N*02301, N*01302, N*0160, and 4 class II proteins; Bovine lymphocyte (BoLA) class II 16, 22, 3, 24 proteins. The Copenhagen lab has developed a new assay for peptide binding to class II MHC molecules and tested the bovine molecules in this new assay. The results confirm the bovine proteins work in this assay.
The graduate student from our collaborating lab at University of Vermont conducted analysis of the bovine class I proteins in the Copenhagen lab and has now established the binding characteristics of these eight bovine MHC proteins. These results are presently being applied to the proteins of FMDV, strain A24 using the NetMHCpan algorithm in order to predict T cell epitopes for FMDV.
No technologies were transferred or publications produced in FY 2013.