2011 Annual Report 1a.Objectives (from AD-416) Goals of this agreement are to 1. Develop disinfectant efficacy test methods that will be acceptable to USDA and EPA for the purpose of conducting research studies. 2. Conduct research studies to determine the effectiveness of selected antimicrobial pesticides for inactivating priority FAD agents on various surfaces. 3. Test the inactivation of less virulent agents by antimicrobial pesticides to determine their suitability for use as surrogates for FAD agents in disinfectant efficacy testing. FY 2009 Amendment includes additional goals: 4. Develop advanced disinfectant efficacy test methods that will be acceptable to USDA and EPA for the purpose of conducting research studies. 5. Conduct research studies to determine the influence that different test conditions will have on the effectiveness of selected antimicrobial pesticides for inactivating FAD agents on various surfaces. 6. Determine the disinfectant application parameters under which optimal effectiveness of selected disinfectants against priority FAD agents can be achieved. 7. Test optimized disinfectant methodologies and optimized application parameters under conditions similar to those encountered in the field. 1b.Approach (from AD-416) Testing of antimicrobial disinfectant chemicals and products to inactivate FAD agents will be accomplished through: 1. Implementation of a quality assurance plan, 2. Literature review of current decontamination practices and selection of decontamination technologies, 3. Development and selection of standardized laboratory techniques to measure the efficacy decontamination products. Laboratory studies to determine the efficacy of selected decontamination products against FAD agents conducted in three phases, differentiated by priority. Testing and evaluation of potential surrogates for FAD agents. FY 2009 amendment Advanced Study Plan will be accomplished through: 4. Evaluation of efficacy testing inclusive of test design, statistical analysis, use-dilutions, application methods, data tables, observations and conclusions. Candidate disinfectants will be applie on inoculated environmental surfaces and tested for efficacy against selected FAD agents inclusive of Foot-and-Mouth Disease virus, Classical Swine Fever virus and African Swine Fever virus. 5. Candidate disinfectants will be applied on inoculated environmental surfaces and tested for efficacy against select FAD agents inclusive of Avian Influenza virus, Rift Valley Fever virus and Newcastle Disease virus. 6. Continuation of disinfectant efficacy testing of selected disinfectant candidates against select FAD agents inclusive of Rinderpest virus, Venezuelan Equine Encephalitis virus, Japanese Encephalitis virus, African Horse Sickness virus, and Akabane Virus. 7. Candidate disinfectants will be applied on inoculated environmental surfaces and tested for efficacy against select FAD agents against surrogate pathogens that could potentially be used in place of FAD agents. USDA, PIADC will conduct basic and applied research under BSL-3 conditions to determine the effectiveness of antimicrobial chemical/products against FAD agents. EPA will provide quality control requirements and expertise in antimicrobial chemical/products analysis. 3.Progress Report 1.Using the standardized techniques for porous and non-porous surface disinfection previously developed, we completed the generation of data to determine and confirm the concentrations of citric acid and sodium hypochlorite required to disinfect Foot-and-Mouth Disease Virus (FMDV), African Swine Fever Virus (ASFV) and Classical Swine Fever Virus (CSFV) on porous and nonporous surfaces, 2. Extended this data by performing dose-response and time course assays to demonstrate concentration and temporal effects of selected disinfectants on virus survival, 3. Tested the inactivation of FMDV and ASFV dried on wood using citric acid and sodium hypochlorite, 4. Determined the efficacy of sodium carbonate against FMDV and ASFV dried on steel and, 5. Determined that bovine rhinovirus type 1 (BRV-1) is not an acceptable surrogate for FMDV in our disinfection assays. Final experiments to complete the project were finished. We delivered a standard operating procedure for porous surface virus disinfection to EPA, based on the data we generated for FMDV. Using this SOP, we generated additional disinfection data determining and confirming the effectiveness of selected chemicals to disinfect ASFV and FMDV dried on wood surfaces.