2010 Annual Report 1a.Objectives (from AD-416) Goals of this agreement are to 1. Develop disinfectant efficacy test methods that will be acceptable to USDA and EPA for the purpose of conducting research studies. 2. Conduct research studies to determine the effectiveness of selected antimicrobial pesticides for inactivating priority FAD agents on various surfaces. 3. Test the inactivation of less virulent agents by antimicrobial pesticides to determine their suitability for use as surrogates for FAD agents in disinfectant efficacy testing. FY 2009 Amendment includes additional goals: 4. Develop advanced disinfectant efficacy test methods that will be acceptable to USDA and EPA for the purpose of conducting research studies. 5. Conduct research studies to determine the influence that different test conditions will have on the effectiveness of selected antimicrobial pesticides for inactivating FAD agents on various surfaces. 6. Determine the disinfectant application parameters under which optimal effectiveness of selected disinfectants against priority FAD agents can be achieved. 7. Test optimized disinfectant methodologies and optimized application parameters under conditions similar to those encountered in the field. 1b.Approach (from AD-416) Testing of antimicrobial disinfectant chemicals and products to inactivate FAD agents will be accomplished through: 1. Implementation of a quality assurance plan, 2. Literature review of current decontamination practices and selection of decontamination technologies, 3. Development and selection of standardized laboratory techniques to measure the efficacy decontamination products. Laboratory studies to determine the efficacy of selected decontamination products against FAD agents conducted in three phases, differentiated by priority. Testing and evaluation of potential surrogates for FAD agents. FY 2009 amendment Advanced Study Plan will be accomplished through: 4. Evaluation of efficacy testing inclusive of test design, statistical analysis, use-dilutions, application methods, data tables, observations and conclusions. Candidate disinfectants will be applie on inoculated environmental surfaces and tested for efficacy against selected FAD agents inclusive of Foot-and-Mouth Disease virus, Classical Swine Fever virus and African Swine Fever virus. 5. Candidate disinfectants will be applied on inoculated environmental surfaces and tested for efficacy against select FAD agents inclusive of Avian Influenza virus, Rift Valley Fever virus and Newcastle Disease virus. 6. Continuation of disinfectant efficacy testing of selected disinfectant candidates against select FAD agents inclusive of Rinderpest virus, Venezuelan Equine Encephalitis virus, Japanese Encephalitis virus, African Horse Sickness virus, and Akabane Virus. 7. Candidate disinfectants will be applied on inoculated environmental surfaces and tested for efficacy against select FAD agents against surrogate pathogens that could potentially be used in place of FAD agents. USDA, PIADC will conduct basic and applied research under BSL-3 conditions to determine the effectiveness of antimicrobial chemical/products against FAD agents. EPA will provide quality control requirements and expertise in antimicrobial chemical/products analysis. 3.Progress Report The work performed under this agreement encompasses research to develop and evaluate methodologies for determining the efficacy of antimicrobial disinfectant chemicals/products against FAD agents and to provide data, analyses and study conclusions necessary to identify effective antimicrobial chemicals/products to be used during outbreaks of FAD agents. New test methods will be developed and validated so that reliable efficacy data can be produced and used to identify effective antimicrobial chemicals/products that can be employed against highly pathogenic FAD agents. In FY 2010, we completed the following: 1.Using the standardized techniques for non-porous surface disinfection developed in FY 2009, generated data to determine and confirm the concentrations of citric acid and sodium hypochlorite required to disinfect FMDV, ASFV and CSFV. 2. Extended this data by performing dose-response and time course assays to demonstrate concentration and temporal effects of selected disinfectants on virus survival. 3. Developed a methodology for the recovery of FMDV from a porous surface (wood) and tested the inactivation of FMDV dried on wood using citric acid and sodium hypochlorite. 4. Determined successful neutralization conditions for another disinfectant, sodium carbonate, and have preliminary results suggesting it is an effective disinfectant against FMDV but not ASFV. To date, the major accomplishments of this project include: 1. a Quality Assurance Project Plan (QAPP) incorporating elements of a Quality Management Plan (QMP) was written and approved by USDA and EPA. The QAPP, included Standard Operating Procedures (SOPs) for the Basic Study Plan and for all aspects of laboratory work. 2. Development and selection of Standardized Laboratory Techniques to measure the efficacy of decontamination products. In FY 2011 we will finish experiments to complete the project, as funding has been cancelled for FY2011 and beyond. The primary focus is to deliver a standard operating procedure for porous surface virus disinfection to EPA, based on the data we have generated for FMDV and forthcoming data that will be generated with ASFV. Using this SOP, we will generate disinfection data to determine and confirm the effectiveness of selected chemicals to disinfect ASFV and FMDV dried on wood surfaces. Three manuscripts are planned; 1. Review article based on the literature review deliverables of the SOW will be completed. 2. Manuscript describing the nonporous surface disinfection SOP and the data for FMDV, ASFV and CSFV that has been generated will be completed. 3. Manuscript describing the porous surface disinfection SOP and the data generated for ASFV and FMDV will be completed. Activities in this project were monitored through email and telephone exchange. The EPA sent auditors to Plum Island to evaluate the team members on the quality workflow of the standard operating procedures, which resulted in a very favorable review. Two members of the team were invited to the biannual EPA Disinfection Conference in April 2010 where we presented a talk covering our current virus disinfection methodology and our recent data.