ASSESSMENT OF GENETIC EROSION IN FUNCTIONAL DIVERSITY IN BRASSICA GERMPLASM COLLECTIONS
2010 Annual Report
1a.Objectives (from AD-416)
The objectives of this cooperative research agreement are to:.
1)validate a set of polymorphic markers (SSR or SNPs) located around candidate genes or gene networks important in adaptive differentiation; and.
2)assess variation in these loci across inventories of diverse Brassica accessions.
1b.Approach (from AD-416)
The first part of the project makes use of the extensive genomic annotation in Arabidopsis thaliana and looks for homologous loci within Brassica species. These objectives dovetail with ongoing work that includes identifying key functional loci and assessing allelic variation at these loci in progeny of crosses phenotyped in controlled environment. We will begin development of genotyping approaches at these loci in a wider pool of accession that have undergone multiple rounds of regeneration.
Genetic characterization of diversity within and among 25 targeted accessions of Brassica nigra from Turkey, Ethiopia and India. We have begun this process by surveying and evaluating 35 SSR loci isolated from Brassica napus. We have another 45 SSR loci to evaluate. We have completed the initial round of phenotypic evaluations and crossing experiments in the greenhouse at CSU. These data have yielded important information on the quantitative trait variation among these accessions and self-incompatibility that is shown to be common but not universal. Finally we have initialed a multi location set of observation plots to catalog the range in phenological and phenotypic variation that exists within and among these accessions. Both the neutral and quantitative trait variation are important supporting data to collect before we examine the variation for particular functional traits. ADODR monitoring is performed by e-mails, phone calls, and on-site visits (co-located with CSU).