Location: Grape Genetics Research
2009 Annual Report
Phenotype one mapping population relevant to winegrape breeding: 1. Analyzing key primary and secondary metabolites of the Horizon x Illinois 547-1 mapping population developed by Dr. Bruce Reisch at Cornell. A total of 400 samples (2 replicates x 2 years x 100 lines) will be analyzed.
Analyze phenotypic data and carry out marker-trait association analysis: 1. Characterizing properties of the phenotypic trait variation investigated. 2. Collaborating with Dr. Ed Buckler and his group to carry out marker-trait association analysis. 3. Validating association analysis results with linkage mapping data.
The second step was to analyze the Vitis berry samples collected from the field. Each berry sample was ground in liquid nitrogen to a fine powder after removing the seeds. Anthocyanins and other phenolics were extracted using 0.5g berry tissue and analyzed using the method described above. We have finished the HPLC runs on the berry samples collected in the fall of 2008, including 306 samples of wild Vitis species, 78 samples of hybrids, and 359 samples of V. vinifera. Data analysis is in progress. In the coming year, similar analysis will be repeated for these samples. These results will provide an assessment of the range of variation of these important grape quality traits in the USDA Vitis germplasm, which will in turn accelerate the development of grape varieties with superior quality.
Monitoring activities for this project includes meetings, emails, phone and annual reports. Also, the ADODR makes site visits to the cooperator’s laboratory and vineyard sites. Any significant deviation from the agreed approaches and methodologies is discussed.