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United States Department of Agriculture

Agricultural Research Service

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Research Project: INOCULATION OF ALMOND ROOTSTOCK WITH SYMBIOTIC ARBUSCULAR MYCORRHIZAL FUNGUS FOR IMPROVEMENT OF NUT YIELD AND FOOD SAFETY

Location: Foodborne Toxin Detection and Prevention

2011 Annual Report


1a.Objectives (from AD-416)
1) Determine if there is value in adding arbuscular mycorrhizal (AM) fungi inoculum, particularly at planting. 2) Determine if pre-plant fumigation impacts the extent and nature of AM fungal populations in the soil and is this of consequence? 3) Characterize the AM fungi populations present on field grown nursery stock vs. potted plants at the time of planting and the resulting tree performance.


1b.Approach (from AD-416)
Nonpareil almond trees on Nemaguard rootstock will be planted in Spring 2008 at USDA Parlier. Plot strips will be fumigated in the fall of 2007 and non-fumigated plot strips will be used as comparison. An assessment of the extent and nature of AM fungi populations present in the soil/residual roots after fumigation but before planting. At time of planting and before any inoculation, there will be an assessment of the extent and nature of AM present on tree roots. Tree performance data to be collected will include: (1 Trunk circumference: initial and final yearly. (2 Annual pruning weights; Nutrient status-characterize nutritional deficiencies if and when symptoms arise. 3. At end of trial: Whole tree top weight, trunk diameter, etc. Annual assessments will be made of the extent and nature of AM fungal populations on tree roots.


3.Progress Report

Soil borne arbuscular mycorrhizal (AM) fungus forms a symbiotic relationship with most plants. The AM symbiosis improves plant phosphorus, nitrogen, and mineral nutrition. Evidence also suggests the symbiosis provides protection of the plant against pathogens and improves plant water relations. The status of AM fungal population in almond orchards is not well understood. The purpose of this study is to determine if specific agronomic practices, such as pre-plant fumigation and inoculation with AM fungus, have an impact on AM fungal populations and subsequent tree performance.

Almond trees planted for this project are now in their fourth year of growth in fumigated and non-fumigated soils. Root and soil samples around the trees will be monitored for root colonization and soil phosphate and microbial populations for several years.

Fifty soil samples were collected from fumigated and non-fumigated almond trees. Roots were sorted out for DNA extraction using PowerSoil DNA isolation kit. Primers from AM fungal ribosomal genes were used for generating polymerase chain reaction (PCR) fragments. Fragments were cloned and subjected to DNA sequencing. Sequencing data were blasted to a National Center for Biotechnology Information (NCBI) database for facilitating AM fungal identification. Almond plants inoculated with green house grown and commercial AM fungi showed colonization.

The ADODR monitored this project through site visits, emails, and phone calls. The goal of the specific cooperative agreement is to develop practical applications of AM fungi to almond orchards. AM fungal colonization of almond roots may improve the health and drought resistance of the plants, which indirectly affects aflatoxin contamination of edible nuts. The research contributes directly to Objective 4 of the in-house project.


Last Modified: 4/18/2014
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