1a.Objectives (from AD-416)
To study gene expression in cultivated sweet potato and its diploid relatives.
1b.Approach (from AD-416)
Selected tissues from sweet potato and its diploid relatives will be collected
and mRNA isolated and converted to cDNA to be sequenced by 454 technology at ARS
The Genomics and Bioinformatics Research Unit does not have the necessary facilities to grow plant material under different growing conditions nor does it have expertise in sweet potato physiology, whereas the scientists at Alcorn State University (ASU) specialize in sweet potato. Various sweet potato tissue samples have been prepared or collected at the Biotech Center, ASU for the project. The following sweet potato (SC1149) tissues, generated under various treatments, are being prepared for total ribonucleic acid (RNA) extraction, 1)leaves, vine, fibrous roots, pencil roots, young storage roots from plants grown in low or high Nitrogen media in a hydroponic system, which are either treated or not treated with the vine-growth inhibitor, Apogee; 2)leaves, vine, fibrous roots, pencil roots, young storage roots from plants grown in the field, which are treated or not treated with Apogee; and 3)leaves, vine, fibrous roots, pencil roots, young storage roots from plants grown in pots and subjected to various stress (e.g. drought and cold, etc.). This project was developed to understand gene expression in sweet potato. Also included was tissue-culture I. tenuissima (2x) for making BAC library.
Participants met at both locations several times during the year and have held conference calls for discussions on the project.