Gene Expression Analysis in Sweet Potato
2009 Annual Report
1a.Objectives (from AD-416)
To study gene expression in cultivated sweet potato and its diploid relatives.
1b.Approach (from AD-416)
Selected tissues from sweet potato and its diploid relatives will be collected
and mRNA isolated and converted to cDNA to be sequenced by 454 technology at ARS
The Genomics and Bioinformatics Research Unit does not have the necessary facilities to grow plant material under different growing conditions nor does it have expertise in sweet potato physiology, whereas the scientists at Alcorn State University specialize in sweet potato. This project was developed to understand gene expression in sweet potato. mRNA was isolated from various tissues of sweet potato (I. batatas) and its two progenitor diploid species(I. tenuissima and I. trifida) grown under different conditions for eventual analysis via pyro-sequencing. Plant material of one of the progenitor diploid species of cultivated sweet potato was cultured under sterile conditions to provide DNA for construction of a Bacterial Artificial Chromosome (BAC) library.
Participants met at both locations several times during the year and have held conference calls for discussions on the project.