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United States Department of Agriculture

Agricultural Research Service

Research Project: KARNAL BUNT COOPERATIVE RESEARCH

Location: Hard Winter Wheat Genetics Research Unit

2013 Annual Report


1a.Objectives (from AD-416):
The objectives of this cooperative research are:.
1)develop and test wheat lines with resistance to KB; and.
2)develop and test molecular markers for resistance to KB.


1b.Approach (from AD-416):
Wheat experimental lines have been developed by crossing adapted varieties with different sources of resistance to KB. Wheat lines will be tested for resistance to KB in the field in India. Genetic mapping populations and near isogenic lines will be developed to identify and validate molecular markers for KB resistance genes. Molecular marker genotyping will be done in India and the USA. Data will be analyzed cooperatively.


3.Progress Report:

This is the final report of the project entitled “Karnal Bunt Cooperative Research” with Punjab Agricultural University.

Resistance to KB was identified in several experimental hard red winter wheat lines. One line with moderate resistance to KB was released as cultivar ‘TAM 401’ by Texas A&M University. A doubled haploid mapping population between a source of very high resistance (KRL22) and a susceptible line (WH542) was evaluated over four years at Ludhiana, Punjab, India and for three years at Obregon, Sonora, Mexico. Resistance segregated as a quantitative trait and heritability of resistance was moderately high. This population was also genotyped using the 90K wheat genotyping array. Data are being analyzed to find the locations of resistance genes for Karnal bunt as well as suitable linked molecular markers. A resistance backcrossing program using cultivar ‘Jagger’ as the recurrent parent was carried to the fourth backcross generation. A derivative of Jagger with very good agronomic type and very good resistance to Karnal bunt was developed.


Last Modified: 10/1/2014
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