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United States Department of Agriculture

Agricultural Research Service

Research Project: KARNAL BUNT COOPERATIVE RESEARCH PROGRAM
2010 Annual Report


1a.Objectives (from AD-416)
The objectives of this cooperative research project are: .
1)Organize and coordinate the Winter Wheat Karnal Bunt (KB) Screening Nursery (WWKBSN) in collaboration with CIMMYT and PAU;.
2)Obtain, increase, and distribute new KB-resistant wheat germplasm lines to regional breeding programs; and.
3)Make crosses between known KB-resistant germplasm lines and elite adapted winter wheat materials from regional wheat breeding programs;.
4)Select adapted KB-resistant lines for germplasm release and cultivar development.


1b.Approach (from AD-416)
Resistant varieties are an important component of an integrated strategy to control KB. The use of resistant varieties is the most economical, sustainable, and long lasting means of control. Dedicated KB screening nurseries exist in collaborating research programs at CIMMYT in Mexico and Punjab Agricultural University (PAU) in India. These nurseries will be used to test experimental wheat breeding lines and mapping populations from the USA for resistance to KB. Known KB-resistant spring wheat lines will be obtained and crossed with locally adapted varieties. Resulting lines will be tested for resistance in the KB nurseries. Field selection will be done at multiple locations for agronomic type.


3.Progress Report

The Eighth Winter Wheat Karnal Bunt Screening Nursery (WWKBSN) was prepared in August, 2009 from entries received from various winter wheat breeding programs. It included replicated entries of many lines that have shown good resistance in past years. It also included a doubled haploid population for mapping KB resistance genes. The nursery was sent for evaluation to CIMMYT in Mexico and to Punjab Agricultural University (PAU) in India. In addition, reserve seed from resistant entries from previous years were also tested. Cooperators at each organization artificially inoculated each entry with Karnal Bunt in early spring, 2010. A site visit to Obregon, Mexico was done in April, 2010 to rate materials for resistance. A few materials with consistently good levels of KB resistance have been identified through the cooperative screening of the WWKBSN at CIMMYT and in India. These include some backcross lines with the cultivar Jagger and some synthetic-derived lines in a Karl92 background. None of these materials have shown immunity, but their level of resistance to KB is probably sufficient to give protection at the field level. Next season, promising advanced lines will be retested to confirm resistance.

Progress on this agreement is monitored by regularly discussing program goals, approaches, and results (teleconference, email) and by reviewing annual accomplishments reports.


Last Modified: 12/21/2014
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