1a.Objectives (from AD-416):
1) Develop genetic crosses of selected isolates of P. graminis, and P. striiformis;. 2)Make collections of rust fungi in Israel;. 3)Establish and monitor field trap plots for wheat rust in Israel; and. 4)characterize spore types and biology of selected rust fungi.
1b.Approach (from AD-416):
Telia of selected isolates will be used to infect appropriate alternate host and genetic crosses will be made from resulting pycnia. Pure lines resulting from the crosses will be developed and characterized on selected host plants. Collections of rust fungi will be made from selected sites in Israel. Biological material will be produced and collected from infection of appropriate hosts. Experiments will be performed to test selected inhibitors on early steps in mating. Spore morphology and life-cycle biology will be characterized for selected rust samples.
Development of genetic resources for mapping in the wheat stem rust fungus (Puccinia graminis f. sp. tritici) continued. Selfs and crosses were made using six different P. graminis isolates. Study on the different nuclear states of different spore stages of the wheat stripe rust pathogen (P. striiformis f. sp. tritici) has begun.