1a.Objectives (from AD-416)
1) Develop genetic crosses of selected isolates of P. graminis, and P. striiformis;. 2)Make collections of rust fungi in Israel;. 3)Establish and monitor field trap plots for wheat rust in Israel; and. 4)characterize spore types and biology of selected rust fungi.
1b.Approach (from AD-416)
Telia of selected isolates will be used to infect appropriate alternate host and genetic crosses will be made from resulting pycnia. Pure lines resulting from the crosses will be developed and characterized on selected host plants. Collections of rust fungi will be made from selected sites in Israel. Biological material will be produced and collected from infection of appropriate hosts. Experiments will be performed to test selected inhibitors on early steps in mating. Spore morphology and life-cycle biology will be characterized for selected rust samples.
Trap nurseries were established at three locations in Israel for monitoring wheat rust pathogens. Collections of Puccinia graminis (wheat stem rust pathogen) were made and characterized. Crosses were performed to develop materials for genetic analysis of P. graminis. In addition biological materials (pycnial and aecial) of P. graminis and P. triticina were generated for RNA-seq analysis and sent to the Cereal Disease Laboratory. Initiated the development of methods for collecting basidiospores of P. graminis and P. triticina. Began testing a wide range of different hosts in order to identify the sexual host of new type of leaf rust of wheat found in Israel. Conducted inoculation studies of Berberis with P. striiformis.
Regular e-mails and an annual visit were used to monitor progress of this project.