1a.Objectives (from AD-416)
1) Develop genetic crosses of selected isolates of P. graminis, P. recondita, P. triticina and P. triticina/P. persistens;. 2)make collections of rust fungi in Israel;. 3)characterize proteins involved in mating in rust fungi; and. 4)characterize spore types and biology of selected rust fungi.
1b.Approach (from AD-416)
Telia of selected isolates will be used to infect appropriate alternate host and genetic crosses will be made from resulting pycnia. Pure lines resulting from the crosses will be developed and characterized on selected host plants. Collections of rust fungi will be made from selected sites in Israel. Biological material will be produced and collected from infection of appropriate hosts. Experiments will be performed to test selected inhibitors on early steps in mating. Spore morphology and life-cycle biology will be characterized for selected rust samples.
Genetic crosses were performed with North American isolates of Puccinia graminis to generate biological materials for genetic analysis. Trap nurseries were set up at three locations in Israel and collections of P. graminis (wheat stem rust), P. striiformis (wheat stripe rust) and P. triticina (wheat leaf rust) were made. Biological materials were generated for characterization of proteins involved in mating type experiments and sent to collaborators. Collections of P. recondita were made and isolates were purified. Method for determining DNA content from urediniospores was improved.
Regular e-mails and an annual scientific meeting were used to monitor progress of this project.