2012 Annual Report
During FY 2012, multiple experiments were performed. For transmission studies, the high efficiency particle air (HEPA) filtered flexible film isolation unit was validated for conducting and containing high pathogenicity avian influenza (HPAI) virus when used for infected chicken processing studies. Studies indicated that negative-ionizing air samplers were more efficient at recovering HPAI virus in air when using a dry sample collector than a liquid filled sample collector. In looking at the initial step in chicken processing, using a standard open barrel method for slaughter produced high concentrations of HPAI virus in the air while reduced quantities of airborne virus were produced when using a Hala pot with a sliding lid for initial kill step. A cyclone sampler was validated to replace the negative-ionizing air sampler as a more efficient instrument to detect airborne virus. The cyclone sampler determined that the majority of the airborne virus was contained in large respiratory droplets, but some was present in aerosolized particles. In examining slaughter methods, the standard open-barrel method produced the most airborne virus, with increased reductions in airborne virus using a cook pot with lifting lid, a cook pot with sliding lid and modified plastic bucket. These data indicate that alternative methods can be used in developing countries for household slaughter to decrease human risk for HPAI virus infections.