STUDY OF INFLUENZA AND NEWCASTLE DISEASE VIRUSES CIRCULATING IN ASIA
Exotic and Emerging Avian Viral Diseases Research Unit
2012 Annual Report
1a.Objectives (from AD-416):
The objective of this cooperative reearch project is to jointly study avian influenza viruses and paramyxoviruses (Newcastle disease) of poultry and wild birds that potentially can cross between birds and humans.
1b.Approach (from AD-416):
The tasks to be performed will include collection and analysis of field samples (cloacal/tracheal swabs, sera and organ samples) obtained from apparently healthy and diseased animals. Viruses will be isolated in cell cultures and embryonating chicken eggs. Antigenic and serological studies as well as molecular-epidemiological evaluation of newly isolated influenza and Newcastle disease virus strains will be done. Genomic analysis of influenza and Newcastle disease viruses isolates will include sequencing (full length or partial) of surface glycoproteins and internal genes and phylogenetic studies.
This project is related to objective 1 of this in-house project: Characterize variant and emerging avian influenza viruses in live poultry markets and commercial production systems.
Some strains of avian influenza virus have been zoonotic agents necessitating joint research between veterinary medicine and human health to solve the problems including the H5N1 high pathogenicity avian influenza (HPAI) virus. The objective of this cooperative research project is to jointly study avian influenza viruses and paramyxoviruses (Newcastle disease) of poultry and wild birds that potential can cross between birds and humans. In order to assist the Co-operator as an international reference laboratory and research institute on avian influenza in wild birds, during FY2012, ARS provided to the Co-operator serological and virological typing reagents for H14, H15 and H16 low pathogenic avian influenza viruses and one H5N1 low pathogenic avian influenza virus. This completes the complement of diagnostic reagents needed as a reference laboratory for identify and subtype avian influenza viruses isolated from wild birds.