2013 Annual Report
1a.Objectives (from AD-416):
The objective of this cooperative reearch project is to jointly study avian influenza viruses and paramyxoviruses (Newcastle disease) of poultry and wild birds that potentially can cross between birds and humans.
1b.Approach (from AD-416):
The tasks to be performed will include collection and analysis of field samples (cloacal/tracheal swabs, sera and organ samples) obtained from apparently healthy and diseased animals. Viruses will be isolated in cell cultures and embryonating chicken eggs. Antigenic and serological studies as well as molecular-epidemiological evaluation of newly isolated influenza and Newcastle disease virus strains will be done. Genomic analysis of influenza and Newcastle disease viruses isolates will include sequencing (full length or partial) of surface glycoproteins and internal genes and phylogenetic studies.
This research is directly related to inhouse objective 1 - Characterize variant and emerging avian influenza viruses in live poultry markets and commercial production systems; inhouse objective 3 - Identify genetic and biological determinants of avian influenza virus susceptibility and resistance in avian species; and to inhouse objective 4 - Improve existing diagnostic tests and testing strategies for avian influenza virus surveillance, detection, and recovery from disease outbreaks.
Some strains of avian influenza virus have been zoonotic agents necessitating joint research between veterinary medicine and human health to solve the problems including the H5N1 high pathogenicity avian influenza (HPAI) virus. The objective of this cooperative research project is to jointly study avian influenza viruses and paramyxoviruses (Newcastle disease) of poultry and wild birds that potential can cross between birds and humans. During FY2013, the co-operator collected a total of 5864 avian samples from the wild birds of the orders of Anseriformes, Charadriiformes, and Passeriformes, as well as poultry, from various sites located in Eastern Siberia. In addition, they screened 7069 wild bird cloacal samples. In total 31 avian influenza viruses and 2 Newcastle disease viruses were isolated. This indicates avian influenza and Newcastle disease viruses continue to circulate on in wild waterfowl in Siberia.