2008 Annual Report 1a.Objectives (from AD-416) Identify and map molecular markers and/or genes that control the expression of specific economically important traits. Determine the loci and/or closely linked molecular markers associated with plant resistance to downy mildew using QTL analysis. Determine the loci and/or molecular markers associated with the production of beta acids in hop lupulin glands. Identify loci and/or molecular markers associated with short internode trait in hop. Develop germplasm with improved agronomic characteristics and brewing quality with combined resistance to two fungal pathogens using traditional breeding procedures. Develop predictive models for hop downy mildew and powdery mildew linked to management thresholds as components of a systems approach to pest management. Derive and validate algorithms relating weather and inoculum parameters to appearance of and infection risk by Pseudoperonospora humuli. Develop and validate PCR assays and DNA extraction protocols to monitor and detect airborne inoculum of Pseudoperonospora humuli. Develop a predictive model to estimate incidence of powdery mildew in cones and identify management threshold based on economic functions of crop loss and incorrect management decisions. Identify and develop integrated production strategies optimizing the control of both powdery mildew and biological control of arthropod pests. 1b.Approach (from AD-416) Development and implementation of systems approaches incorporating genetic and IPM methods to reduce production costs and labor inputs. This project uses a systems approach including hop germplasm improvement for production, brewing characteristics and disease resistance through an understanding of genomics, epidemiology, and genetic mechanisms contributing to resistance to downy mildew (Pseudoperonospora humuli) and powdery mildew (Podosphaera macularis).Conventional and molecular breeding techniques will be used, PCR methodologies, GC/MS spectrophotometric, and HPLC techniques. Germplasm will be released for public use. IPM approaches will simultaneously be developed to control these diseases on cultivars with contrasting levels of genetic resistance. Previous Projects: 5358-21000-019-00D (Exp 4/01). Replacement for 5358-21000-030-00D (Exp 03/04). FY04 Program Increase $201,306. Add 1 SY. Replacing 5358-21000-035-00D (5/08). 3.Progress Report This project, approved through the OSQR process, replaces CRIS project 5358-21000-035-00D effective 6/16/08 (Normal Progression). Milestones addressed in FY 2008 are listed in the final Annual Report for the terminated project. Report of progress for the fiscal year is documented in the final Annual Report of project 5358-21000-035-00D. Experiments are being conducted as outlined in the project plan. This research addresses National Program 301, Component 2. 4.Accomplishments 1. This project, approved through the OSQR process, replaces CRIS project 5358-21000-035-00D effective 6/16/08 (Normal Progression). Major accomplishments for the fiscal year are documented in the final Annual Report for 5358-21000-035-00D. 5.Significant Activities that Support Special Target Populations None. 6.Technology Transfer Number of Other Technology Transfer 1