2010 Annual Report 1a.Objectives (from AD-416) To maintain and increase seed of advanced potato breeding selections from the Beltsville Potato Breeding Program. To put into tissue culture and rid of virus said selections. 1b.Approach (from AD-416) Culture plantlets on various media to induce organogenesis and rooted plantlets. Apply ELISA tests to detect potential latent virus infections. Nodal cuttings will be used to increase plantlets and produce microtubers. Plantlets, microtubers, and minitubers will be planted in the field for seed increases of pathogen-free stocks of advanced clones for further evaluation. 3.Progress Report Three USDA bred potato varieties (Amey, Harley Blackwell, and Peter Wilcox) were maintained in tissue culture. An advanced selection, B1992-106, was established in tissue culture and greenhouse micropropagation initiated. This agreement is a critical link in making high quality seed of advanced selections and recently released varieties from the USDA Potato Breeding Program in Beltsville, MD available to the potato industry for production and utilization. Progress is being monitored by e-mail and telephone.