2012 Annual Report
Animal Waste Management Research Unit, Service: D Aquatic Animal Health Research Unit, Service: D Biological Control of Pests Research Unit, Service: D Catfish Genetics Research Unit, Service: D,B,G, Commodity Utilization Research Unit, Service: D Coastal Plains Soil, Water, and Plant Research Center, Service: D Corn Host Plant Resistance Research, Service: D,B Cotton Fiber Bioscience Research Unit. Service: D, G Crop Genetics and Breeding Research, Service: D, B, M Crop Genetics Research Unit, Service: D, G, B, M Crop Germplasm Research, Service: G Dale Bumpers National Rice Research Center: D, M Endemic Poultry Viral Disease Research Unit, Service: D,B Food Processing and Sensory Quality Research Unit, Service: D Genetics and Precision Agriculture Research, Service: D Natural Products Utilization Research Unit, Service: D, National Peanut Research Lab, Service: D, G, B, M Poultry Microbiological Safety Research, Service: d Southern Insect Management Research Unit, Service: D,B Soybean Genomics and Improvement, Service: D, B Subtropical Horticulture Research Station, Service: G, Sugarcane Field Station, Service: D,G, Sugarcane Research Unit, Service: G Sunflower Research Unit, Service: D Tropical Agriculture Research Station, Service: B,D,G,M Tropical Plant Genetic Resources and Disease Research Unit: G
DNA marker development, validation, and utilization, is an important output component of the unit. This is often in conjunction with its service component and impact most of the major crops within the Mid-South Area like cotton and soybean. In the past year work was conducted on yam and tepary bean. Yam is a very important carbohydrate crop for many world agricultural systems. DNA markers were processed on a yam mapping population related to anthracnose resistance. Tepary bean is a plant native to southwest part of the USA and thus has important traits related to drought and heat tolerance. It is also known to be an excellent source of disease resistance genes for crop species like common bean. DNA markers were developed, validated and tested on small germplam collection illustrating they can be used on the larger U.S. collection. Another major output of the unit is DNA sequencing. In January a project was started to sequence the ends of 100,000 Bacterial Artificial Chromosomes (BACs) which will be useful for sequence the genome of upland cotton. The use of next generation DNA sequencing has greatly increased in the past year for cotton and insects. It is anticipated that this trend will only increase as more samples for other species are submitted, e.g. peanut, maize and soybean.
Arias, R.S., Molin, W.T., Ray, J.D., Peel, M., Scheffler, B.E. 2011. Isolation and characterisation of the first microsatellite markers for Cyperus rotundus. Weed Research. 51:451-460.
Nelson, J.C., Jodari, F., Roughton, A.I., McKenzie, K.S., McClung, A.M., Fjellstrom, R.G., Scheffler, B.E. 2012. QTL mapping for milling quality in elite western U.S. rice germplasm. Crop Science. 52:242-252.
Thomson, M.J., Zhao, K., Wright, M., Mcnally, K.L., Rey, J., Tung, C., Reynolds, A., Scheffler, B.E., Eizenga, G.C., Mcclung, A.M., Kim, H., Ismail, A.M., Ocampo, M., Mojica, C., Reveche, M., Dilla, C.J., Mauleon, R., Leung, H., Bustamante, C., Mccouch, S.R. 2011. High-throughput SNP genotyping for breeding applications in rice using the BeadXpress platform . Molecular Breeding. 29:875-886.
Dean, D., Wadl, P.A., Trigiano, R., Wang, X., Klingeman, W., Ownley, B.H., Rinehart, T.A., Scheffler, B.E. 2011. Screening and characterization of eleven novel microsatellite markers from Viburnum dilatatum. HortScience. 46(11):1456-1459.
Nelson, J., Oard, J.H., Groth, D., Utomo, H., Jia, Y., Liu, G., Moldenhauer, K.K., Correa-Victoria, F.J., Fjellstrom, R.G., Scheffler, B.E., Prado, G.A. 2011. Sheath-blight resistance QTLs and in japonica rice germplasm. Euphytica. 184:23-24.
Solomon, W.L., Kanter, D.G., Walker, T.W., Baird, III, G.E., Scheffler, B.E., Lanford, L.S., Shaifer, S. 2012. Registration of "Rex" Southern Long-Grain Rice. Journal of Plant Registrations. 6(1).
Trigiano, R.N., Wadl, P.A., Dean, D., Hadziabdic, D., Scheffler, B.E., Runge, F., Telle, S., Thines, M., Ristaino, J., Spring, O. 2012. Ten polymorphic microsatellite loci identified from a small insert genomic library for Peronospora tabacina. Mycological Society of America. 104(3):633-640.
Arias, R.S., Blanco, C.A., Portilla, M., Snodgrass, G.L., Scheffler, B.E. 2011. First microsatellites from Spodoptera frugiperda (Lepidoptera: Noctuidae) and their potential use for population genetics. Annals of the Entomological Society of America. 104(3):576-587.
Hadziabdic, D., Wadl, P.A., Boggess, S.L., Scheffler, B.E., Windham, M.T., Trigiano, R.N. 2011. Development and characterization of sixteen microsatellite loci for Geosmithia morbida, the causal agent of thousand canker disease in black walnut (Juglans nigra). Conservation Genetics. 4(2):287-289.
Wadl, P.A., Dattilo, A.J., Scheffler, B.E., Trigiano, R.N. 2011. Development of microsatellite loci for the endangered species Pityopsis ruthii (Asteraceae)1. American Journal of Botany. 0:e1-e4.
Yu, J., Fang, D.D., Kohel, R.J., Ulloa, M., Hinze, L.L., Percy, R.G., Zhang, J., Chee, P., Scheffler, B.E., Jones, D.C. 2012. Development of a core set of SSR markers for the characterization of Gossypium germplasm. Euphytica. 187(2):203-213.